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Part of the reason for the differences of opinion is simply the ambiguity of the OP's post title and content. To be perfectly clear, yes the nut quality and cut absolutely affects fretted chords and notes per the title of the OP. This is where TG, myself, and a few others are coming from.
As for tone itself, per the content of the OP , don't expect a life changing experience between two nuts of different materials if all else is identical. Yes, there can be a difference. Drop several nut blanks of various materials on a hard surface and you can hear the difference. This difference as a percentage of the composite instrument tone will be very small however. This is where Ron is coming from.
Another way to summarize all this is to say that a good nut won't necessarily make a terrible guitar great..... but a poor nut can make a great guitar terrible. Since most guitars fall somewhere in the middle, it's safe to say that you can never realize the full potential of a guitar without having a well cut nut, regardless of material.
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