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Hello old_picker,
The 5U4 rectifier has more voltage drop than the GZ34, the voltage at the plates will go down. You'll need to check bias and adjust as necessary for proper tube dissipation. Also make sure your 5v windings on the PT can handle the additional load, the GZ34 uses 1.9 amps and the 5U4 uses 3.0 amps.
Ken
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